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After washing 3 times, cells were incubated in serum-free medium with 1 mM CaCl 2 for 1 h. Because bacterial pathogens such as P. As a general rule we do not censor any content on the site. After incubation, resins were washed 3 times with buffer containing mM NaCl and 40 mM imidazole. C , D Coimmunoprecipitation.
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